Submitted by: Yue Zheng

Category: Microscopy

Contributors: Li Fang, Maryam Mahmoodi, Hao-Cheng Gao, Fan Xu, Han Zhao & Fang Huang

Creation: Photoactivation localization microscopy (PALM) imaging of a living COS-7 cell stably expressing a HaloTag on reticulum 4(Rtn4) protein stained with PA-JF549-Halo. The raw single molecule blinking data was taken on a custom-built TIRF imaging system using a 405 nm laser for photoactivation and a 561 nm laser for excitation. Data was analyzed and reconstructed using the INSPR algorithm, and the localizations in 80 seconds were plotted through a jet (64) color map.

Caption: The structural changes and fast movements of ER tubules are clearly resolved at the nanometer scale of spatial resolution and seconds level of temporal resolution. The color from blue to red indicates the actual time from 0 to 80 seconds. The scale bar at the lower right is 500 nm.