Single-molecule localization microscopy is a powerful tool for visualizing organelle structures, interactions and protein functions in biological research. However, whole-cell and tissue specimens challenge nanoscopy methods' achievable resolution and depth. As imaging depth increases, photons emitted by fluorescent probes are scattered and aberrated, resulting in image artifacts and rapidly deteriorating resolution.

Huang proposed a method of point spread function retrieval for three-dimensional nanoscopy. The system involves multiple components, including a 3D nanoscopy imaging device, an encoder that uses the images to provide point spread functions (PSFs), and a processor that receives PSFs and provides 3D locations of the molecules in a system. The processor uses an in situ PSF retrieval algorithm (INSPR) that iteratively forms 3D PSF stacks for single molecule patterns until the detected and retrieved PSFs match. This system provides limit-achieving precision and uncompromised fidelity through whole cells and tissues.

Congratulations to all Purdue University researchers across all campuses and academic disciplines, who received a patent on their intellectual property from the U.S. Patent and Trademark Office in April.

Most of these innovations are available to license and bring to market. Visit the Purdue Innovates Office of Technology Commercialization’s website to learn more about these and other available innovations.

Related publications: Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell Tissue Specimens