Submitted by: Ishita Mukadam

Category: Microscopy

Creation: Cortical bone slices were fixed, sectioned, and stained with Alcian blue to visualize glycosaminoglycan (GAG) content. Imaging was performed using a Zeiss brightfield microscope.

Caption: This image shows a rat tibia bone slice stained with Alcian blue under a brightfield microscope. The stain highlights glycosaminoglycans(GAGs) within the bone matrix, which play a crucial role in regulating bone hydration and structural integrity. By staining the bone slices, we can visually quantify the bone matrix, which is valuable in the context of biomarker chronic kidney disease (CKD).