Submitted by: Julia Walsh

Category: Microscopy

Creation: Glass samples were coated with various substances, clockwise from top left: Chitosan, Gelatin, Collagen, and 2-MethacryloyloxyethylPhosphorylcholine, and then placed in an Albumin-GFP solution to allow for fluorescent protein adsorption, which allows some defects and texturing on the surface to be visualized based on the protein’s binding properties. Images were collected using an inverted confocal fluorescent microscope.

Caption: Coating presence creates changes in the chemistry of a surface that can change how it interacts with nearby molecules, specifically their ability to bind to it. Here, both defects and textures become visible by allowing fluorescent albumin to bind to coated surfaces. The coatings used, clockwise from top left, were Chitosan, Gelatin, Collagen, and 2-Methacryloyloxyethyl Phosphorylcholine.