Abstract:

This presentation will describe efforts in our lab to develop microphysiological systems (MPSs) -such as organs-on-a-chip or Transwell cultures and organoids- of the lung. The talk will start with microfluidic small airway models that combine in-channel air-liquid interface culture with liquid plug propagation and rupture under conditions of surfactant dysfunction. Transwell-96 based air-blood barriers model will be described that facilitates virus infections studies and also promises to accelerate studies of effects of air pollution on lung inflammation. The presentation will also describe airway organoids that have an inverted, apical-out geometry. These Organoids with Reversed Biopolarity (ORBs) are used in 384 well plates in a single-organoid-per-well format to test drugs and disease physiology. The airway ORBs are infected with high yields with multiple SARS-CoV-2 strains; the Omicron variant showing the highest viral replication and the Delta variant inducing a higher inflammatory signature. The airway ORBs also predict anti-viral drug efficacy correctly where conventional 2D cultures give false signals. Time permitting studies using human intestinal organoids and mammary organoids that mimic early-stage breast cancer progression will also be briefly covered.

Biography:

Prof. Shuichi Takayama’s research interests started with bioorganic synthesis at the University of Tokyo and Scripps Research Institute.  Subsequently he pursued postdoctoral studies in bioengineered microsystems at Harvard University as a Leukemia and Lymphoma Society Fellow. He spent 17 years at the University of Michigan in the Biomedical Engineering Department and Macromolecular Science and Engineering Program, then moved to the Wallace H. Coulter Department of Biomedical Engineering at the Georgia Institute of Technology and Emory School of Medicine in the summer of 2017. He is an associate editor of Integrative Biology and recipient of the Pioneers of Miniaturization Prize and Steven A. Denning Faculty Award for Global Engagement. He is Director of the Nakatani RIES Program which promotes international undergraduate student internships between the US and Japan.

~ BME Host: Estelle Park ~

Zoom Link: https://purdue-edu.zoom.us/j/93634966285

Students registered for the seminar are expected to attend in-person.

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