Abstract

Engineering heterogenous multicellular tissue with native complexity remains one of the holy grails of regenerative medicine and basic biological research. As success in this regard would yield powerful bioengineered constructs useful in functional transplantation, high-throughput drug screening, and fundamental biology investigation, research efforts in our lab have centered around developing and implementing tools to spatiotemporally customize living cell function both from the “outside–in” and from the “inside–out”. In this talk, I will discuss some of our group’s recent successes in reversibly modifying the chemical and physical aspects of synthetic cell culture platforms with user-defined and grayscale control, regulating cell-biomaterial interactions through user-programmable Boolean logic, engineering microvascular networks that span nearly all size scales of native human vasculature (including capillaries), irreversibly photoassembling bioactive proteins within living cells, and driving biomolecular condensate formation using de novo-designed proteins. Results will highlight our ability to modulate intricate cellular behavior including stem cell differentiation, protein secretion, and cell-cell interactions in 4D. 

Biography

Dr. Cole A. DeForest is the Weyerhaeuser Endowed Professor in the Departments of Chemical Engineering and Bioengineering, the Associate Chair and Graduate Program Director of Chemical Engineering, the Director of Education of the Molecular Engineering & Sciences Institute, as well as a core faculty member of the Institute for Stem Cell & Regenerative Medicine at the University of Washington (UW) where he began in 2014. He received his B.S.E. degree from Princeton University in 2006, majoring in Chemical Engineering and minoring in Material Science Engineering and Bioengineering. He earned his Ph.D. degree under the guidance of Dr. Kristi Anseth from the University of Colorado in Chemical and Biological Engineering with an additional certificate in Molecular Biophysics. His postdoctoral research was performed with Dr. David Tirrell in the Divisions of Chemistry and Chemical Engineering at the California Institute of Technology (Caltech). He has published ~90 peer-reviewed articles, including as the corresponding author for those appearing in Nature Materials, Nature Chemistry, Nature Chemical Engineering, Nature Chemical Biology, Advanced Materials, JACS, PNAS, Science Advances, Nature Reviews Materials, and Nature Reviews Bioengineering. Dr. DeForest has received numerous research awards and honors including the Society for Biomaterials’ Mid-Career Award (2025) and Young Investigator Award (2020), NIH Maximizing Investigators’ Research Award (MIRA R35, 2020), NSF CAREER Award (2017), and many others. Notably, he has also been recognized for excellence in teaching and was awarded the UW Presidential Distinguished Teaching Award (2016), given annually to a single Assistant Professor across all of the UW, as well as the UW Outstanding Undergraduate Research Mentor Award (2025), given annually to a single engineering professor at UW. His research has been supported through fellowships and grants from the National Science Foundation, the National Institutes of Health, the Defense Advanced Research Projects Agency, and the US Department of Education.