New Course: ABE 560/BME 521 - Biosensors: Fundamentals and Applications

Engineering Faculty Document No

 

TO:         Engineering Faculty

FROM:  The Faculty of Agricultural and Biological Engineering

RE:          New Course

 

The faculty of the Department of Agricultural and Biological Engineering has approved the following new course.  This action is now submitted to the Engineering Faculty with a recommendation for approval.

 

ABE 560/BME 521 – Biosensors:  Fundamentals and Applications

 

Sem. 2, Class 3, cr 3.

 

Prerequisites:  BIOL 295E, CHM 116, and MA 266 or equivalents.        

 

Description:

An introduction to the field of biosensors and an in-depth and quantitative view of device design and performance analysis.  An overview of the current state of the art to enable continuation into advanced biosensor work and design.  Topics emphasize biomedical, bio-processing, environmental, food safety, and bio-security applications. 

 

Reasons:

A number of students across campus are engaged in biosensors research.  The specific research areas of the students vary substantially from fundamental materials chemistry to microbiology to device design and are being applied to agricultural, food process, biomedical, environmental, and bio-security applications.  This class is intended to  formalize the basic fundamentals of biosensors and to provide a broad understanding of the current state of the art.  While this need is immediate, we project a long term need for the course, as biosensors are predicted to remain an important research topic in the future.  In addition, as the technology advances, we are likely to see an increased role of biosensors in industry.

 

 

 

 

 

Vince F. Bralts

Head, Department of Agricultural and Biological Engineering

 

 

 


 

ABE 560/BME 521 – Biosensors:  Fundamentals and Applications

 

Sem. 2, Class 3, cr 3.

 

Prerequisites:  BIOL 295E, CHM 116, and MA 266 or equivalents. 

 

Description:

An introduction to the field of biosensors and an in-depth and quantitative view of device design and performance analysis.  An overview of the current state of the art to enable continuation into advanced biosensor work and design.  Topics emphasize biomedical, bio-processing, environmental, food safety, and bio-security applications. 

 

Suggested reference and/or textbooks:

Written materials will be provided from various sources including class notes, peer-reviewed literature (primarily from the journals Biosensors & Bioelectronics and Analytical Chemistry) and selected excerpts from text references including:

 

Bilitewski, U. and Turner, A.P.F. 2000.  Biosensors for Environmental Monitoring.  Harwood

    Academic Publishers, The Netherlands.  ISBN: 90-5702-449-7.

Ligler, F.S. and Rowe Taitt, C.A. 2002.  Optical Biosensors: Present & Future.  Elsevier, The

    Netherlands. ISBN: 0-444-50974-7.

Yang, V.C. and T.T. Ngo. 2000. Biosensors and Their Applications. Kluwer

    Academic/Plenum Publishers, New York, NY. ISBN: 0-306-46087-4.

 

Course Learning Objectives:

At the end of the course the students will be able to:

1.      define the fundamental components of any biosensor,

2.      define the major performance characteristics of any biosensor and
design an experiment to measure that characteristic ,

3.      evaluate a sensor based on standard performance criteria and
appropriateness for an application,

4.      given a specific biosensor application, identify the key design
criteria and suggest and an appropriate biosensor approach which is most
likely to meet those design criteria,

5.      compare the relative advantages and disadvantages of the major
approaches to biosensor design,

6.      communicate the most relevant challenges facing the biosensor
research field and given a particular challenge suggest a reasonable
approach to finding a solution to the challenge,

7.      describe what challenges are shared among and what challenged are
unique to the major biosensor application areas.


Syllabus:

Week

 

Topics Covered

Activities

1

Introduction to 

    Biosensors

course overview, class survey, definitions, motivation, biological inspiration, types of sensors, target analytes, various recognition, signals, and device types, history of field

Assign journal papers for student presentations

2

Basic Design

    Considerations

calibration, dynamic Range, signal to noise, sensitivity, selectivity, interference

 

3

Recognition /

    Transduction

enzyme sensors

Student  presentations

4

Recognition /

    Transduction

affinity sensors: antibodies, oligo-nucleotides

measuring binding in affinity sensors, SPR, quartz crystal microbalance, FRET

Hands-on Lab Experience - SPR

5

Recognition /

    Transduction

membrane protein sensors: ion channels, receptors

Exam 1

6

Recognition /

    Transduction

whole cell sensors – bacteria, yeast, mammalian cells

 

7

Recognition /

    Transduction

non-biological and bio-mimicry: molecularly imprinted polymers, non-biological organic molecules, electro-chemiluminescence, pH sensors, artificial receptors

 

8

Immobilization

immobilization: adsorption, encapsulation -(hydro-gel, sol-gel glass, etc.), covalent attachment, diffusion issues

 

9

Device Integration

optical fiber sensors, planar wave-guides

Hands-on Lab Experience – Optical Fibers

10 -11

Device Integration

micro-scale and nanoscale: BioMEMS, nanowires, quantum dots, magnetic beads, PEBBLE sensors

Exam 2

12

Special

    Considerations

measuring complex samples, multi-analyte detection, continuous measurements, reagentless biosensors

 

13

Special Considerations

implantable sensors, biocompatibility issues

 

14

Applications

agricultural, food safety, food processing : state of the field, market potential, unique design criteria and needs, current sensors in use

 

15

Applications

biomedical applications, bio-security, environmental : state of the field, market potential, unique design criteria and needs, current sensors in use

Exam 3

Student presentations

16

 

 

Final Exam

 


Grading Policy:          Attendance                               60 pts

                                    Exam 1                         60

                                    Exam 2                         60       

                                    Exam 3                         60

                                    In Class Presentation*            100

                                    Final Exam                             100

                                                                                  440 pts

 

* Each student will present a journal paper to the class.  The presentations will be given throughout the semester and will be related to the lecture topics for that week.

 

 

Grading Scale:           A         > 87 %            

                                    B          78 – 87 %

                                    C         68 – 77 %

                                    D         58 – 68 %

                                    F          < 58 %