The Lin Research Group is located at Purdue University’s urban campus in Indianapolis. The laboratory is located at the Innovation Hall (IO204) with a total wet lab area of 1,300 sq. ft. Major equipment includes:

Chemical synthesis:

Two 6-ft fume hoods with vacuum/gas dual manifold; Buchi rotary evaporator; Branson® S-450 Sonifier digital ultrasonic processor and acoustic sound enclosure; CEM Discover 2.0 microwave synthesizer; Labconco bench-top lyophilizer; Flammable storage refrigerator and freezer.

Hydrogel preparation and biofabrication:

Photopolymerization equipment and light sources (e.g., UV and visible light lamps, Omnicure S2000 UV spot light curing systems); UVP Crosslinker CL-3000L; Cellink BIOX 3D bioprinter; Cellink Lumen X+ 3D bioprinter; 5 syringe pumps.

Materials characterization:

Anton Paar MCR 102e rheometer with DMA accessories; Bohlin CVO digital rheometer with UV and visible light cure modules; CellScale MicroTester G2; PreSens Microx 4 fiber optic oxygen transmitter, sensors, and VisiSens oxygen imaging system.

Cell culture & imaging:

Cell culture

One 9-ft, two 6-ft tissue culture laminar flow hoods; Hypoxia incubator chamber and flow meter; Five sets of CO2 incubators; Liquid nitrogen and cryogenic storage; Countess® II FL automated cell counter.

Imaging

BioTek Lionheart FX automated live cell imager; Cellink Cellcyte X automated live cell imager; ECHO Discover Rebel microscope; Nikon Ti inverted optical microscope with epifluorescence attachment and a CCD camera; Oxford Instruments Andor BC43 benchtop confocal microscope.

Cell and molecular biology:

Apparatus for gel electrophoresis and Western Blotting; Bio-Rad T100 thermal cycler; AirClean® AC600 Series PCR Workstations with HEPA-filtered air & UV light irradiation; BioTek 50 TS plate washer; Eppendorf epMotion 5070m liquid handling workstation; Nanodrop 2000; DS-11 FX+ Microvolume/Cuvette Spectrophotometer and Fluorometer; Synergy HT multi-mode microplate reader; Molecular Device SpectraMax iD5 multi-mode microplate reader; QuantStudio 3 real-time PCR machine. Namocell Pala single cell sorter.

Other standard equipment:

Chromatography refrigerator, 4°C refrigerator, -20°C freezer, and -80°C freezer; Heat ovens, vacuum oven, and desiccators; Top load balance, analytical balance; pH meters; Stirring hotplates; Bench-top microcentrifuge, orbital shakers, and orbital shaker incubator; Bench-top centrifuges and microcentrifuges; Orbital shakers.