[BNC-grads-list] NSAC Coffee Hour talk tomorrow on Cryo TEM

Fri Nov 3 14:54:21 EDT 2017

Just a reminder that we have the NSAC coffee hour at 3:30 pm today. Please
see the details below:

On Thu, Nov 2, 2017 at 11:19 AM Sajid Choudhury <sajidmc at purdue.edu> wrote:

> Dear All,
>
> This year, Jacques Dubochet, Joachim Frank, and Richard Henderson— won the
> Nobel prize in Chemistry for their work on developing *Cryo-Electron
> Microscope*. I am happy to announce that our next NSAC Coffee hour topic
> would cover work by Christopher on Cryo TEM. Please see below for title and
> abstract of his talk.
>
> *Also, we have GSOGA funding, so we will have special snacks for
> tomorrow's coffee hour.*
>
>
> 
> *What: NSAC Coffee Hour talk*
> **
> *When: Friday, November 3rd, 2017 3:30 pm*
> *Where: BNC Atrium*
> *Speaker: **Christopher J Benjamin*
> Topic: Development of Techniques Using Novel Bioorganic Materials for
> Protein Structure Determination
>
> ABSTRACT: Single-particle reconstruction has grown significantly with
> the improvements in various data collection and computational strategies
> including CTF fitting, the use of vitrified samples and the utilization of
> ultra-sensitive direct electron detectors. Although these improvements have
> contributed significantly to the recent evolution of 3D reconstruction
> analysis, the way samples are prepared for electron microscopy has remained
> largely unchanged. We report the development of TEM grids that are modified
> with non-fouling coatings bearing surface grafted nitrilotriacetic acid
> substituents that promotes specific capture of protein targets for
> high-resolution TEM analysis. The utilization of these grids for specific
> adsorption of the targeted protein onto the grid surface results in
> well-controlled surface concentration enhancements and a days-to-minutes
> reduction in time required for the preparation of a purified sample for
> cryoEM analysis from an *E. coli* expression system. The selective and
> reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude
> lysates, as well as purified nanodisc-solubilized his-malFGK2, on these
> NTA-modified grids with an exceptionally low level of adsorption by
> non-target proteins, has been observed. Our data illustrate the utility of
> these grids for selective capture from complex mixtures,
> detergent-solubilized membrane protein isolates, and expression systems
> yielding low copy numbers of the desired target in a manner that is
> well-suited for single particle reconstruction analysis.
>
>
> Also, please feel free to sign up for the Spring coffee hour slots in the Google
> sheet
> <https://mailtrack.io/trace/link/7fe51a6c721d6d80a12f5662bf5f73ed9de80dc0?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=89dfc57c0f626d8d> by
> clicking here
> <https://mailtrack.io/trace/link/6f43651d269a915008cf6b4751984f0a88b2c4f8?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=756856486ac0b824>
> .
>
> [image: Inline image 1]
>
> Thank you.
>
> Sajid Choudhury
> President
> Nanotechnology Student Advisory Council (NSAC)
> https://engineering.purdue.edu/NSAC/
>
> <https://mailtrack.io/trace/link/f64a86a3462f87cc1469f2cb5e83ddbf9bc95065?url=http%3A%2F%2Fsecure-web.cisco.com%2F1jBfhmTyJyjIvvoQGZyMPpPPtCZwljBCnvQtKcDtrJPrkuNeXGkLs1fb65CJqsCL3dd2_IDq54FmdQzDC9mWQp_N6BJW8-RCDhP3i98B1vyX1a0isF4vAiGzw_SgO1fDGr0w6BEl-TTAeKwxbmPOgeFreDRvJhvBNCyrLviPIdYzCCpgvSlInmD_YBv6fJ1Br9hBlpfHx-2iSRci8EW5Bii2IXzhEQeq4bQ5GkwIcarWXQny14-UsabONl4tjV0ViONTSQNWQymdM03pt7TbMSPgtmJCIIRmhV3lzh3yspUZPQR_ZErp-YuUS7Yma-bqaQc_pl3mv9GHvJR062FgJ8NdN5A-IPYlzjlYKatyFpc0%2Fhttp%253A%252F%252Fgoog_878781996%252F&userId=2154587&signature=9fa76079e6de911b>
>
>
> <https://mailtrack.io/trace/link/f64a86a3462f87cc1469f2cb5e83ddbf9bc95065?url=http%3A%2F%2Fsecure-web.cisco.com%2F1jBfhmTyJyjIvvoQGZyMPpPPtCZwljBCnvQtKcDtrJPrkuNeXGkLs1fb65CJqsCL3dd2_IDq54FmdQzDC9mWQp_N6BJW8-RCDhP3i98B1vyX1a0isF4vAiGzw_SgO1fDGr0w6BEl-TTAeKwxbmPOgeFreDRvJhvBNCyrLviPIdYzCCpgvSlInmD_YBv6fJ1Br9hBlpfHx-2iSRci8EW5Bii2IXzhEQeq4bQ5GkwIcarWXQny14-UsabONl4tjV0ViONTSQNWQymdM03pt7TbMSPgtmJCIIRmhV3lzh3yspUZPQR_ZErp-YuUS7Yma-bqaQc_pl3mv9GHvJR062FgJ8NdN5A-IPYlzjlYKatyFpc0%2Fhttp%253A%252F%252Fgoog_878781996%252F&userId=2154587&signature=9fa76079e6de911b>
> and
>
> Ph.D. Candidate,
> School of ECE, Purdue University
>
>
>
> On Tue, Oct 24, 2017 at 1:04 PM, Sajid Choudhury <sajidmc at purdue.edu>
> wrote:
>
>> [image: Inline image 1]
>> We have NSAC Coffee hour this week, Mohamed Hagag from Peroulis group
>> will give a talk on his research.
>>
>> **
>>
>>
>>
>>>
>>
> ‌
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>
-- 
Sajid M Choudhury. (Sent from gmail iphone)
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