[BNC-grads-list] NSAC Coffee Hour talk tomorrow on Cryo TEM

Thu Nov 2 11:19:35 EDT 2017

Dear All,

This year, Jacques Dubochet, Joachim Frank, and Richard Henderson— won the
Nobel prize in Chemistry for their work on developing *Cryo-Electron
Microscope*. I am happy to announce that our next NSAC Coffee hour topic
would cover work by Christopher on Cryo TEM. Please see below for title and
abstract of his talk.

*Also, we have GSOGA funding, so we will have special snacks for tomorrow's
coffee hour.*

*What: NSAC Coffee Hour talk*
**
*When: Friday, November 3rd, 2017 3:30 pm*
*Where: BNC Atrium*
*Speaker: **Christopher J Benjamin*
Topic: Development of Techniques Using Novel Bioorganic Materials for
Protein Structure Determination

ABSTRACT: Single-particle reconstruction has grown significantly with the
improvements in various data collection and computational strategies
including CTF fitting, the use of vitrified samples and the utilization of
ultra-sensitive direct electron detectors. Although these improvements have
contributed significantly to the recent evolution of 3D reconstruction
analysis, the way samples are prepared for electron microscopy has remained
largely unchanged. We report the development of TEM grids that are modified
with non-fouling coatings bearing surface grafted nitrilotriacetic acid
substituents that promotes specific capture of protein targets for
high-resolution TEM analysis. The utilization of these grids for specific
adsorption of the targeted protein onto the grid surface results in
well-controlled surface concentration enhancements and a days-to-minutes
reduction in time required for the preparation of a purified sample for
cryoEM analysis from an *E. coli* expression system. The selective and
reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude
lysates, as well as purified nanodisc-solubilized his-malFGK2, on these
NTA-modified grids with an exceptionally low level of adsorption by
non-target proteins, has been observed. Our data illustrate the utility of
these grids for selective capture from complex mixtures,
detergent-solubilized membrane protein isolates, and expression systems
yielding low copy numbers of the desired target in a manner that is
well-suited for single particle reconstruction analysis.

Also, please feel free to sign up for the Spring coffee hour slots in
the Google
sheet
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[image: Inline image 1]

Thank you.

Sajid Choudhury
President
Nanotechnology Student Advisory Council (NSAC)
https://engineering.purdue.edu/NSAC/
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and

Ph.D. Candidate,
School of ECE, Purdue University

On Tue, Oct 24, 2017 at 1:04 PM, Sajid Choudhury <sajidmc at purdue.edu> wrote:

> [image: Inline image 1]
> We have NSAC Coffee hour this week, Mohamed Hagag from Peroulis group
> will give a talk on his research.
>
> **
>
>
>
>>
>
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