TR Stratton, RE García, BM Applegate, JP Youngblood “Application of a High Throughput Bioluminescence-Based Method and Mathematical Model for the Quantitative Comparison of Polymer Microbicide Efficiency.” Biomacromolecules. 10(5):1173-1180, 2009.
Quaternized poly(4-vinyl pyridine)-based copolymers are known to be effective against a wide range of bacteria and possess biocompatible properties. Extensive testing of a wide range of copolymers is necessary to further explore and enhance the biocidal properties. However, testing is hampered by labor-intensive bacteria testing techniques. The present paper presents a new testing method, based on bioluminescent reporter strains to enable fast evaluation of bactericidal properties. The reported method enables us to create real-time characterization of bacteria behavior with far less labor than required through traditional testing methods. A mathematical model was also developed to characterize the change in bacteria populations exposed to biocides and to enable the quantitative comparison of minimum bactericidal concentrations.