[BNC-all] 3D3C Newsletter - February 2017

Mon Mar 13 10:26:50 EDT 2017

Dear All,

Below please find the eighth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center.  The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news).

The four sections in the newsletter are:
3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue
3D in focus – this section presents the current work on a specific 3D cell culture model or technique
3D in publications – this section brings a collection of recent publications on 3D cell culture
3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture

The newsletter will be available every two months.  If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe.
Please contact me if you have questions.

Yours Sincerely,

Tim Kwok
Facility Manager
3D Cell Culture Core (3D3C) Facility
Birck Nanotechnology Center
Purdue University

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Volume 8, February 2017

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3D at Purdue

3D in Focus

3D in Publications

3D in Meetings

3D at Purdue

The Savran Laboratory focuses on the development of novel platforms for rare cell detection and analysis. Especially in the context of the disease of cancer, isolation of rare cells from blood samples of patients is extremely important as it offers a glimpse into the tumor using a simple blood test. One important downstream application for such methods is testing of drugs in a personalized manner. One can isolate the tumor cells of a particular patient and find out which drugs are effective in killing these cells before trying drugs directly on the patient. However tumor cells are found in blood samples only in extremely small amounts. This is a challenge because one normally needs a lot of cells to test sufficient combinations and dosages of drugs. It is therefore important to culture the isolated cells and increase their number. While culturing the cells, it is important to mimic conditions that the cells experience in their natural environment as much as possible so that the resulting tests can correlate with realistic outcomes. This can best be achieved with 3D culture conditions where cells are suspended in a manner that surrounds them with tissue-like milieu, as opposed to 2D methods where cells attach to a flat surface, a condition that is not natural.

We are collaborating with Drs. Lelièvre and Kwok to develop novel methods to culture single rare cells in a manner that allows high throughout analysis and testing. We expect that these studies will enhance our understanding of heterogeneity and pave the way to personalized therapy.

Professor Çağrı A. Savran
School of Mechanical Engineering
School of Biomedical Engineering (by courtesy)
School of Electrical and Computer Engineering (by courtesy)

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3D in Focus

Osteoarthritis is one of the leading causes of chronic disability for elderlies.  One of the possible causes of the disease is synovial inflammation by macrophage activation.  The objective of the article highlighted in this newsletter was to build and validate a 3D coculture system for osteoarthritis inflammation through paracrine interactions between chondrocytes and macrophages.

Featured article: Satyavrata Samavedi, Patricia Diaz-Rodriguez, Joshua D. Erndt-Marino, and Mariah S. Hahn.  A Three-Dimensional Chondrocyte-Macrophage Coculture System to Probe Inflammation in Experimental Osteoarthritis Tissue.  Tissue Engineering: Part A 2017, 23: 101-114.

In the transwell system, normal chondrocytes (NCs) cultured in the upper chamber and activated macrophages (AMs) cultured in the bottom chamber were both placed in a 3D environment by encapsulation within poly(ethylene glycol) diacrylate hydrogels. Increased levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines that are associated with early stages of osteoarthritis were observed in NCs cells in the coculture system. Such increases were also detected in osteoarthritic chondrocytes (OACs) in coculture with AMs, although the levels of MMPs and proinflammatory cytokines were already enhanced in OACs due to the natural disease process.  The OACs cultured in the presence of nonactivated macrophages demonstrated a lower increase in levels of MMPs and proinflammatory cytokines, indicating that AMs may intensify the abnormal matrix degradation and cytokine secretion in OACs. In addition, AMs cocultured with OACs expressed significantly more IL-1b and VEGF-A compared to AMs alone, suggesting that OACs may also intensify abnormal activation in macrophages.

Advantages of the proposed model: Despite the primary location of the macrophages in the knee joint within the synovial membrane, in the previously reported models of in vitro osteoarthritis, only the chondrocytes were cultured in a 3D microenvironment; the macrophages were cultured in 2D as a monolayer. The significance of the present model is to culture both chondrocytes and macrophages in a 3D environment. Moreover, the authors of the present report investigated the phenotypic changes in macrophages in coculture with chondrocytes; these cells have not been a focus of study in previous work dealing with osteoarthritis. Furthermore, the use of diseased rather than normal chondrocytes may enable the modeling of the later stages of osteoarthritis progression; this seems an appropriate model for testing potential therapeutic approaches of the disease.

Next possible steps:   Osteoarthritis progression involves many steps and interactions among different cell types and extracellular matrices. The work in the highlighted article, however, examined only the interaction between chondrocytes and macrophages for synovial inflammation. Moreover, models that recapitulate various stages in osteoarthritis are still needed. This work shows like other models before that is possible to obtain interesting answers even when using cell types from two different species (human chondrocytes vs. mouse macrophages).  Future studies using primary human macrophages in the coculture system will be important to confirm the interaction between chondrocytes and macrophages described here.

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 3D in Publications

Recent publications on 3D culture (please click to access the list on our web page
https://nanohub.org/groups/3d3cfacility):

Review<https://nanohub.org/groups/3d3cfacility/news#reviews>

The research articles and reviews are arranged in the following categories:

Scaffold free/Scaffold

Organ/Tissue/Cell

Others

Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids>

Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid>

Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold>

Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel>

Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix>

 Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic>

Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication>

Bone<https://nanohub.org/groups/3d3cfacility/news#bone>

Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow>

Breast<https://nanohub.org/groups/3d3cfacility/news#breast>

Colon<https://nanohub.org/groups/3d3cfacility/news#colon>

Heart<https://nanohub.org/groups/3d3cfacility/news#heart>

Liver<https://nanohub.org/groups/3d3cfacility/news#liver>

Lung<https://nanohub.org/groups/3d3cfacility/news#lung>

Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle>

Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve>

Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate>

 Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells>

Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast>

Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells>

Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells>

Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer>

 Screening<https://nanohub.org/groups/3d3cfacility/news#screening>

3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbioprinting>

 Imaging<https://nanohub.org/groups/3d3cfacility/news#imaging>

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3D in Meetings

Keystone Symposia - Engineered Cells and Tissues as Platforms for Discovery and Therapy
Date: 9th to12th March 2017
Location: Boston, Massachusetts, United States
Website: https://www.keystonesymposia.org/17K1
Contact: Phone: 1 800-253-0685;     Email: info at keystonesymposia.org<mailto:info at keystonesymposia.org>
Organized by: Keystone Symposium

Cell Culture World Congress USA 2017
Date: 23rd to 24th May 2017
Location: San Diego, California, United States
Website: http://go.evvnt.com/68105-0
Contact person: Freya Smale
This event allows research scientists engaged in upstream and downstream bioprocessing to discuss novel techniques and techniques and technologies.
Organized by: Terrapinn USA

The 3rd Annual 3D Cellular Models: Engineering Predictive Preclinical Screening Models
Date: 14th to 15th June 2017
Location: Boston, Massachusetts, United States
Website: http://www.worldpreclinicalcongress.com/3D-Cellular-Models/
Organized by Cambridge Healthtech Institute

The 2nd International Conference "Plant Cells In Vitro: Fundamentals and Applications"
Date: 26th to 27th June 2017
Location: Vienna, Austria
Website: http://viscea.org/index.php/plant-in-vitro
Contact person: Alisher Touraev
The Conference will discuss wide range of modern in vitro plant cell and organ culture technologies, fundamental aspects of plant cell totipotency, differentiation, regeneration, embryogeneisis, and practical applications of in vitro technologies.
Organized by: Vienna International Science Conferences & Events Association (VISCEA)

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