[BNC-all] Nanoseminar: Dr. Chang Lu, Purdue Dept. of ABE, School of Chemical Engineering (By Courtesy), 11/8/06 @ 4 PM BRK 2001 (Microfluidics plus Electricity: New Tools for Single Cell Analysis and Manipulation)

[Annie Cheever]

"Microfluidics plus Electricity: New Tools for Single Cell Analysis 
and Manipulation"

Wednesday, 11/8/06
4:00 PM
Birck Nanotechnology Building, Room  2001
Dr.  Chang Lu, Assistant Professor,
Department of Agricultural and Biological Engineering,
School of Chemical Engineering (By Courtesy)
Purdue University

When heterogeneous cell populations, like those derived from primary 
material, are studied, most sample preparation methods often call for 
both genetic and protein content to be averaged over the tissue 
sample material. However, it is always beneficial to obtain 
information of singe cells to prevent cell subsets and vital 
processes from being overlooked. For example, when cells show an 
all-or-none response to a particular stimulus (bistability), bulk 
analysis yields only the average and does not recognize such 
characteristics. Micro-fluidic devices offer new possibilities for 
analyzing and manipulating cells at single cell level due to their 
micro-scale dimensions. In this seminar, I will discuss some 
micro-fluidics-based techniques developed in my lab for 
electro-poration and electro-fusion of mammalian cells at single cell 
level. We applied these techniques to delivery of impermeant 
molecules and nucleic acids into cells. We also designed a simple 
micro-fluidic system, which carried out rapid electrical lysis and 
chemical analysis of single cells at a maximum rate of 75-85 
cells/minute. Using similar approach, we were able to carry out 
electro-fusion of cells at single pair level. These new tools have 
the potential for applications ranging from drug/gene screening, 
single cell proteomics, anti-body production, cloning mammals to 
cancer immunotherapy.
Dr. Chang Lu joined Purdue University in the fall of 2004 as an 
assistant professor in Agricultural and Biological Engineering, with 
courtesy appointments in Chemical Engineering and in Laboratory of 
Renewable Resources Engineering (LORRE). Dr. Lu obtained his B.S. in 
Chemistry with honors from Peking University in 1998, M.S. and PhD in 
Chemical Engineering in 2001 and 2002, respectively, from University 
of Illinois at Urbana-Champaign. During 2002-2004, he was a 
postdoctoral fellow in the School of Applied and Engineering Physics 
and the Nano-biotechnology Center (NBTC) at Cornell University. He is 
the author of 17 peer-reviewed journal papers and has 2 US patents 
pending. His research group is currently interested in the processing 
and analysis of single cells and single molecules using 
micro/nanofluidics and fluorescence spectroscopy.

Host:  Rashid Bashir Electrical and Computer Engineering Department, 
(66229, bashir at purdue.edu)

SPONSORED BY:
Birck Nanotechnology Center, Bindley Bioscience Center, Discovery 
Park, The NASA Institute for Nanoelectronics and Computing, The 
Network for Computational Nanotechnology, VEECO, NCN Student 
Leadership Council, Department of Chemistry, Department of Physics, 
School of Chemical Engineering, School of Electrical and Computer 
Engineering, School of Mechanical Engineering

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Annie Cheever, Area Secretary                      
School of Electrical and Computer Engineering 
	                                                                 
 
acheever at purdue.edu
			  
Purdue University
Birck Nanotechnology Center, Room 2027
1205 West State Street
West Lafayette, IN  47907-2057

Phone: 765-496-8327     Fax: 765-496-6443     http://www.nano.purdue.edu/  
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