General

Data Location           D:\Experiments\Meenesh\apap_rohd_5_071211

File 1
File Name               apap_rohd_5_071211_raw01.pic
8.3 Name                APAP_R~1.PIC
Size                    11555244 bytes
Created                 2011 Jul 12 16:41
Modified                2011 Jul 12 16:35

File 2
File Name               apap_rohd_5_071211_raw02.pic
8.3 Name                APAP_R~2.PIC
Size                    11555244 bytes
Created                 2011 Jul 12 16:41
Modified                2011 Jul 12 16:35

Total Dataset Size      23110488 bytes
Acquisition Type        XYZ scan
Intensity Resolution    8 bits
Number of Channels      2




Calibration

Lens Name               10x
Magnification           10.00

Width
	Box Resolution        512 pixels
	Pixel Size            0.314 µm
	Box Size              160.662 µm
Height
	Box Resolution        512 pixels
	Pixel Size            0.314 µm
	Box Size              160.662 µm
Z-Depth
	Number of Z-stacks    44
	Origin                77.000 µm
	Step                  5.700 µm




Scanning

Frame Rate              0.11 Hz
Line Frequency          166.0 Hz
Pixel Dwell Time        10.464 µs
Zoom                    5.80 X
Pan X                    0.0 µm
Pan Y                    0.0 µm
Rotation                None
Collection Filter       Direct




Method

Red/Green Dual Label
Sequential
   Sim1
      Simultaneous
      Unlocked:  Detectors, Primary Lasers, Secondary Lasers, Optics, Mixers
      Lasers
         Krypton/Argon
            Neutral Density: ON
            Density:     30.00
            Excitation:  488 + 568nm
            PM Pick-off: 
      Mixers
         PMT 1
            Photon Counting: OFF
         PMT 2
            Photon Counting: OFF
      PMT Detectors
         PMT 1
            Iris:  2.60 mm
            Gain:  1297.000
            Black: 1.70
            Emission Filter: 605/32
            Blocking Filter: Open
            Dichroic Mirror: T1
            Polarization:    OFF
         PMT 2
            Iris:  2.00 mm
            Gain:  979.000
            Black: 1.50
            Emission Filter: 522/35
            Blocking Filter: Open
            Dichroic Mirror: E2
            Polarization:    OFF
      Transmission Detectors
   Red 
      Sequential
      Unlocked:  Detectors, Primary Lasers, Secondary Lasers, Optics, Mixers
      Lasers
         Krypton/Argon
            Neutral Density: ON
            Density:     100.00
            Excitation:  568 nm (Yellow)
            PM Pick-off: 
      Mixers
         PMT 1
            Photon Counting: OFF
      PMT Detectors
         PMT 1
            Iris:  4.00 mm
            Gain:  1500.000
            Black: 1.70
            Emission Filter: 605/32
            Blocking Filter: Open
            Dichroic Mirror: T1
            Polarization:    OFF
      Transmission Detectors
   Green
      Sequential
      Unlocked:  Detectors, Primary Lasers, Secondary Lasers, Optics, Mixers
      Lasers
         Krypton/Argon
            Neutral Density: ON
            Density:     30.00
            Excitation:  488nm (Blue)
            PM Pick-off: 
      Mixers
         PMT 2
            Photon Counting: OFF
      PMT Detectors
         PMT 2
            Iris:  2.00 mm
            Gain:  966.000
            Black: 2.00
            Emission Filter: 522/35
            Blocking Filter: Open
            Dichroic Mirror: E2
            Polarization:    OFF
      Transmission Detectors
