[BNC-all] 3D3C Newsletter - October 2017

[Kwok, Tim]

Dear All,

Below please find the twelfth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center.  The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news<https://secure-web.cisco.com/1--ZQCMqlCOUkLpi0munkkBp4stfTabydGCHayyeghWe3lLMaRxrrZWN2SQ1xatv-eu3WuBB3gPmR4kB3CM9KHUVG1wcWep2TNNrnp15BN5AjkJDvhbr3TR0ECpDncVyJ1fAvQe2VFHgW1fkHuWTRhovSQ5XwI4GYdj-_4vhCNPUJhCOgBVAAUdR-XKZ9TKBbdBVfANT5TtpcKYxBvqEZAm1tJ3CKuqpk-8vAbV-7H8eCzdVBGnX0K3e_DeSHStajphKdpLQi_P21L9OJtuAm3GaCKGZu-5KhxzJIQvL6wkWOs11H4MzoD1_HGn0FgUxqsr6zXnKfRePk24J6m0o4DJ-8NPLQm5f45qKECT4nt64/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews>).

The four sections in the newsletter are:
3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue
3D in focus – this section presents the current work on a specific 3D cell culture model or technique
3D in publications – this section brings a collection of recent publications on 3D cell culture
3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture

The newsletter will be available every two months.  If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe.
Please contact me if you have questions.


Yours Sincerely,

Tim Kwok
Facility Manager
3D Cell Culture Core (3D3C) Facility
Birck Nanotechnology Center
Purdue University

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[https://secure-web.cisco.com/1T3saNnSupk0DX27OLn_KKb16ecnfl6gV-m8RUQnH4pObB_1EbvMiOzYyk84WSXD-1qzSo_D-FQbUlYF1FnWdM70bgFYJuuwgR4T84Qal8UAcXSAoPScJO0RYZH1uUBJvqHM7w0dHFwVfqmnxWs2zpbyPXheY8jd4T7g6Z50_GmqYxV9aRvIHaOa_IAzh4AmRE2d_Ce4F9IlN2K2amMZrO4iolqTACNW1Sf-CN1RQdkXvcxHfzo8C0mxoEzr3MuwVSkyRxdojCxn4UjRG-cxsZLhLxFbTteSFsXvgeOdMMN9eIBZ33_Bmrrd8eRiDghc9wTDbiaitvWHiaIRTydzJJQoVjW6MnQ_jcU6BSHfSrtg/https%3A%2F%2Fgallery.mailchimp.com%2Fb7d6d1ffee56a866e499104cf%2Fimages%2F3e8e050f-67fb-44f6-9c44-aff80a014d62.png]
Volume 12, October 2017



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3D at Purdue

3D in Focus

3D in Publications

3D in Meetings



3D at Purdue



Using Laser Capture Microdissection as an Initial Tool to Investigate

Mechanisms of Plant Root Immunity


Denise Caldwell and Anjali S. Iyer-Pascuzzi

Department of Botany and Plant Pathology


Roots are key not only to plant water and nutrient uptake, but also for plant defense against soilborne pathogens. Nearly 90% of the major plant diseases affecting crops in the United States are caused by soilborne pathogens. A subset of these are root vascular wilts. Soilborne bacteria and fungi that cause vascular wilt diseases first infect plant roots by colonizing water-conducting root tissue known as xylem, which prevents water movement to the shoot, resulting in plant death. The best means of disease control is through resistant varieties, but the mechanisms underlying resistance are not clear. In the Iyer-Pascuzzi laboratory, our goal is to understand the molecular basis of root immunity to pathogenic soil microbes.

We focus on the soilborne bacterium R. solanacearum, the causal agent of bacterial wilt and ranked within the top 10 most destructive plant bacterial pathogens. Root-mediated resistance to R. solanacearum is fundamental to whole plant resistance. We have previously shown that bacteria differentially colonize the root systems of susceptible and resistant plants. Xylem colonization by bacteria is critical to disease progress, and in roots of susceptible plants, bacteria are able to enter and colonize the xylem within 24 hours. At this same time point in resistant plants, bacteria are still found at the outer root cell types. We hypothesize that root cell-type and tissue-specific gene expression is required to prevent R. solanacearum from entering the xylem of resistant plants. To test this, we are using Laser Capture Microdissection (LCM) to harvest specific cell types and tissues in the resistant and susceptible root, including the root cortex and vasculature, at different time points after inoculation with our pathogen. After harvest, we extract RNA and will use RNA-seq to identify genes that are differentially expressed between mock and inoculated root tissues. Using reverse genetics, we aim to identify tissue-specific genes that contribute to root defense mechanisms.

Words from 3D3C: The featured article describes a success story from Dr. Iyer-Pascuzzi’s laboratory of using the LCM system in the 3D3C Facility.

LCM is a technique used for isolating cells from a tissue section, cytological preparation or live cell culture via direct visualization of the cells. Unlike other existing methods, e.g. flow cytometry, LCM isolates the cells without disturbing the in situ environment. The technique will also be useful for research in 3D cell cultures that are composed of heterogeneous cell populations.

The LCM system in the 3D3C is the latest version of Applied Biosystems® ArcturusXT™ Laser Capture Microdissection System.  The system is a unique user-friendly microdissection instrument that combines infrared (IR) laser capture microdissection (LCM) and ultraviolet (UV) laser cutting in one platform. The unique combination of IR laser capture and UV laser cutting permits the collection of selective population, down to a single cell level, of tissues and cells on any slide type and sample preparation, such as thin or thick sections, frozen or formalin-fixed tissues, chromogenic stained or unstained sections, and live cell cultures. Contact Tim Kwok at x46697 or kwokt at purdue.edu<mailto:kwokt at purdue.edu>.



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Steps used in capturing cells with the Laser Capture Microdissection system.  (A) Cross section, (B) selection of the desired cells, (C) laser capture of cells, (D) removal of captured cells, (E) remaining section, (F) captured cells.







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3D in Focus


The formation of new blood vessels in the tumor microenvironment is essential for tumor progression. Cancer associated fibroblasts (CAFs) are a subpopulation of cells that reside within the tumor microenvironment.  CAFs through their secretory and mechanical activities promote the cancerization process by acting on angiogenesis, inflammation and metastasis.  While CAF signaling pathways, e.g.  VEGF, have been extensively studied, the role of CAF-generated mechanical forces in vascularization is poorly understood.  By coculturing endothelial cells (ECs) with CAFs in 3D fibrin gels with embedded magnetic microbeads coated with thrombin, the studies confirm that mechanically perturbing CAFs affects their effect on vascularization.

Mary Kathryn Sewell-Loftin, Samantha Van Hove Bayer, Elizabeth Crist, Taylor Hughes, Sofia M. Joison, Gregory D. Longmore, and Steven C. George. Cancer-associated fibroblasts support vascular growth through mechanical force. Scientific Reports (2017) 7: 12574

3D3C summary of the article: When cocultured with ECs, patient-derived breast CAFs supported the formation of interconnected vascular networks in 3D fibrin gels while normal breast fibroblast (NBFs) did not.  However, CAF conditioned medium only moderately rescued blood vessel growth in NBF samples, demonstrating that soluble factors alone are not sufficient to explain the enhanced blood vessel growth in the presence of CAFs. The CAFs produced significantly higher deformations in the gel compared to NBFs, as shown by gel deformation measurements done by tracking the displacement of beads embedded in the gel. Subjecting the gel containing thrombin-coated magnetic beads to a magnetic field moving at 30 or 50 rpm demonstrated an increase in blood vessel growth in samples containing either ECs, NBFs, or CAFs. Increasing rotation speed to 100 rpm, however, showed no further increase in supported vessel growth. Static magnetic stimulation, for which a magnet was placed adjacent to the gel for the duration of the experiment, induced a slight increase in vascular formation. These studies support that mechanical deformations or perturbations of the extracellular matrix regulate the growth of blood vessels as promoted by CAFs in the tumor microenvironment.

Comments from 3D3C: The conventional way to generate mechanical stimulus in hydrogels is by changing the concentration of polymers or cross-linkers.  The system used by Sewell-Loftin and colleagues provides another method for mechanical stimulation as illustrated here in fibrin gel.  One of the advantages of this magnet-based system is the ability to switch on and off the mechanical force if needed without disrupting the hydrogel, enabling the dissection of kinetics for mechanotransductive pathways in cells.  Even though the present study is focused primarily on CAFs, the same system can also be used for other cell systems such as endothelial cells and other types of fibroblasts (e.g. normal human lung fibroblasts).  The limitation of the present system is that requires using fibrin hydrogel since the interaction between thrombin and fibrin is required. Possibly, in the future, a similar concept can be used with gels made of matrix representing the microenvironment of the cells of interest.





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 3D in Publications


Recent publications on 3D culture (please click to access the list on our web page
https://nanohub.org/groups/3d3cfacility<https://secure-web.cisco.com/1XOVy6CysmV6r_U3i-sTwndP_edvbQhOpObAb2Yu8ByxR8hrHWF812k3GinUgUUSEKhZyvHUzPMbMc1tlegzylzBTDr3EE-fs9uLwhCNK3ymqCmusdx1rkaOV8IAR1V6eSELm2eOpbQ_twPXWPeWkeida_0MToKNWSYNIu_dm2I828eX1aq2mVJI95q6szMjv5rd3e1JTMTM0hX9P4B6my43kjUL574iPAkwI79y7ryytLVUbTCshyHkbGTUGphMcstKIm9knmfV_9zdQF4Si75whcjr4lEaGqMyvxZ-rN9mnrubco56hiEzFDllTeZcyqmyCHUmX_Sgt6KewbY8oVDs4-N6efrNlBupJAOXRpag/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility>):

Review<https://secure-web.cisco.com/1lJ2vrvyml1Pb2jhAww-cco_o0HfHM-wAJcfvDChMTFjByk2BpmoIFV5Rt9kIjc-daTVSi48HiS5ywX2r4mnC8NX7Wg0uF7LJ-nGcTQxhuL9vyhuTiBtQJiolmre2AP0ETAKpf0238AHHTlzbH-AM9f3yZW2uEJepHfIjG-eXVE8g_-KH5aUy6scXKiYPNy2kgoDoBt2aF2ehZtGqHOx9LJ6VGkFWZgbxB5dJ5N1cdGeqeMlcqZVUhc19OOijyIeXhaZC3BMefcaJ9AX5TaCFUtJwcPmr5x_ueXYZqwglzo8vvbzNJZQL53mpMUg2-dIDi6lOROlP5xZezESELDDZHoYLzFN_6JBgUhxT5r4VO5I/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23reviews>

The research articles and reviews are arranged in the following categories:


Scaffold free/Scaffold


Organ/Tissue/Cell

Others


Spheroids<https://secure-web.cisco.com/13Zd53pCqUpKDAYsNEOMdZkojYjwe-F2j3dFHbgoLrfSXNDOK9Ap3r2jz-BzA-ptlRKFczmCcs0YEun5fDUCHC1Wea5x-8o5SnsdNnLTnZYz1XvqEBxIKtDXbQJEkItFC-S2zJKi75l3ZChyZbGsUTxzJ0gT21A2-s372ogkowi2VZIfwhFFZ5wSFSfHIRCnuh_mfE9auba4eIAMTOP3aIpC_-etLPdtxorxopsoUErZbGjnZaMGT6eV0yf6VWOYDA_kw-mknJ_Q9Y61kwHZiNvHFVnEhAimtlzJQ1ElYC6gT5EvnMT_6hJtcPeTuiyLOC9hll_OQJeli1UggdpdU4XXP1UHGaLMx-mflTeXgVwg/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23spheroids>

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Hydrogel<https://secure-web.cisco.com/19f3SFconpXbRClhjks4ypK0NkBVxhxjgnniHzY2PKLqE__LWUcCBd58MjRf8ys1PU3YjdUH9Jvq1SGiNXUweV9EPcmHY2hulkcQjm-LZGnQMqXFwOZ5F48c5ezpyeWL0A3Yjt19fnAA3Evo8TO739kyJtbe2qkWfW4eMST6LuskWQzkzWwQQs7d2y_TO24Yu3vGCEwqbDgIwkfReyfLnAXQOVCJGSkvhPD2kld_dBDpFlcLwzzWYtjqa8WYA5G3Yw29g_PPTdKd7TAx4wELbbywf9h4__ynZR2ASPQcSRqNSGfvB32Anc6mAVJHPpLWUW0hAXd1KIpJK_39drv18QbtCraYuU9smvcOWwJv5ZPo/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23hydrogel>

Matrix<https://secure-web.cisco.com/1yPj8QPLYww0ozgaA54A3y1NIJi8iLb3Za8dtQXuszXwWGlkYd89GPLJ_VmEnk5fY5n71lFOJQcMq892KXF0xdJ_ZvxdgkhuIcJxZcueMFMT_ug0FMoBixR-oQQLdlp_gR5NRma9Tz7SGNWZpjtJobkXR2MlFJ5i72rbLkKjW-iO_clK88927f649N0N5aFSG_b4xkB4IeHOPeOb3XQPZTUhEKKUG46CxfqTLKPlNgAGNqz-r_GAjPBjpeL9h4UfUNX4wjLXOBDr8mI-ZIx5FuC-6ldoFu9ttZLKfsgaOYWmJkd23b6YsLa6-mFlx0HIqXjPyNuAnaBI_iUdW0E_gv7FPDg5horigLE7njIMJpa0/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23matrix>

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Microfabrication<https://secure-web.cisco.com/1I428IyfNn2IxV6fxf4VcnloVbYuFrkuofUUgjx1h7Tdi2p-ArmFmTaEFpHVayWGALYCHdVfKG67X-qXPDWykRZVfytFv4z-7buHIuC-78HOJ470xmkv47fWmBKKRZGFIrhZsuBf2z6aFM4RPwfoY_gt0gP3DVIYjcSFqkDzdIsIuiUhPP1d_GOV4ttDoPD6ODahPVExXLEnHUCT3HGATtkgVTlDVC4ZB27Q1D36GFsJmTtNFyG6XmY3uByrWl1s14kEbw70Mrplv9ZWBM86pBLGoisCBWhj9XOAiVs31DJIy1FvwjvIP-NrW8IWbZT8lWFyVzDmNEcWs748LB7ggCh_C2OGwG1DY5AE-MKIX2RE/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23microfabrication>


Bone<https://secure-web.cisco.com/1F3VkXI67m08FOW_VWeMRDIAupg4u3-J7sYnrGkXp7c3pe4WkedoKFPV2eDvRSz3SpDR_rRYuLuqUz9xI9noBn49UTdev1JdYuvYGUnJ-JAgJSWLK-rgVaCl4ofe3BTfxQ9XRjiQLp96-wxA_77RqtliUbBqMQ02348hLjWdwfUU1jCTWAi5rAmA0NVeOy7tBXrjgAdvFoMGlGsjUaa_96Wem7Q9JDl3oY6aw9B7jvINlMALGa3jdDua8VgZgtV4lD0dL8iOzJhh2sJ82gbKbji5_VAq-PwTQ_Vj9nkeHlah9vcI5dIaS4ZFtXiQ5JJ35t86sM5N0RwTcC9fqJVFd9ecqY-O7AhkbA80O1LIl2Fw/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23bone>

Bone Marrow<https://secure-web.cisco.com/1PZPLPvqE95MUUdEeT578qVgnV_fhCjjDsDr257Rz3M56Lgs0Lo4d4bp2mtFewJOJ66xIFyKz0gZ_BHJ_rNj3Q9mfR_wTiBzR08MejG0fDRJUV01CCkXf4dgAQOjZA0hO8RiKK8st9AChx6z4sbROj7VVjMdfo8vTPL-5C10xWs5dNuDONQqZM4q0hOU0NTiCv9tjuY9htCyKeFgy2ufe_mtXcteUSbPmUu_HrHixjNYufTm9-ZfB3_HUTlE25kjw84bacdQVRMunfjx8blQ97oeH-1TPsLNr19tE80cLxxuwSDw83OXcLnO26SDwLbmfleDm1NZLHE-PgR5CpdsM7TYGRKTjubRMjVBTnigTysg/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23bonemarrow>

Breast<https://secure-web.cisco.com/1pVw7wr81WEbySanUf0vpLL_bSeTXp-sRoc3uoLJ1PAAtQsn0kargBWwdgkaH1Y9X_rSqy0_IooBVWQotblWOSDdkB5d5btrnis6y8DFfZZ7G5WdrPL3mmuxD8UPDjNHtMeAMCDN5j9n0BlTFVLZQvZvP5FGIHyMxRLw2gRHrkKTbO4rXaQJcHcm9Ju1Emy5mYaRn-3Tl-w_ura6IzdcLEw-636zc7nyct1Fi00wKUmhQIux0nEMjna_ZGcvetEtKOr-Mw47SmKWpwsmLli2GtAJbFsC2xbsHkU0djuG4hNQmxkElAcAqslXcytEw-PBprXb569GVx5gE6gicNw2kQPekdSYdvwL8GnE_4GcykXc/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23breast>

Colon<https://secure-web.cisco.com/1zO82eCOI67kNFeFbjXmBSKI1FTIDQbX7w_3mAXZzRrMJUO94pSAAq1uAjHBGkFfC4q-AXH_2dRJOpJE20M8K5-KO63BaDF9VNxWrTcWd152XO0ZMxSjYxKgKQ7ZNlLwaSKx07faPWIHYoVJiHZmBegoypJREcr4E-39vICpfF-RXmXTP_8gWPsKdL9L8DhyBlPdUvA-VKptr5oI8nWaO-q4fAg8d_RC9cxp_ciTp4cvY0J3EwV2W5-s3QVeUZ6VZrGl6LG_9BXeuYBhAXv0_ARusZU2mbtpRFJo2RG_1H6Qz7WtT1HSG6QWxF54a2FFwcQFwh7j8PSelQWbpimTc2Y_zozIp3lP6BEGcxNRZvoQ/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23colon>

Heart<https://secure-web.cisco.com/1IF7d1_mVhPT3dobbBMT_IragJLFFHihmx-wdld2hB0hhtUEjRv31thI6dNBIjUaD19i18lr-tpdQyrhdFV4ERcTbIOHvvoO7bC4N-r6eKS5vOm1C00bKF9LcN-fEFdMt97uro9A7JHmzG92783_L8JH_YOToDyOMifzqc-5zqaB2Vqw_L5woQswzECgUsd7r7D7Ucs6_8Ht-s8VyLnnnJq_sD3ptfVCcGmVSbpmZcl2Ut2CdBvLjBhefzGJH5_FPdcFztj5bdFWKGnP6rUw74hQPgsGjWmiQes1x5y1aeND5L7HEzA2Bl82U7msdUxsP2AGif2Tb2-LsTTfNjnKRs6TinlvvJDo7mJbJRjypjuM/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23heart>

Liver<https://secure-web.cisco.com/1sDhWFnq6Wk3h7Efv-aKWWmN0uvvPzk9_SbcNdT_ZtB1yTguRGyvUTAXQGDU1FmrmM00rriP-5cSjSfZdMrYYwRmHfby_GHfK8CL4iw29hCooHWGH4JA3xAGgIidVObGwJsbIdeYM7qbZOGDYeTZyCr3sjKApjsVgD2dXKq7AT5lApV1WpZwZCp_ZXZrCO_wn2stt4rg_NE3lrO4YdjkqRVUhpJ-3_oq8GuYObuOVWVZ1rg8PBmjnCilCTQK9lWUop99b3Ccs4jVCtmNa0_8LXMQTjkJ2tCaMFuaZWfvAX6Fv7YTFEbJ1UhsmI1LvZHUPTG1qbZTEWKulYtoEPqx-gq0jNWTxH9H5HIak6OcjXO0/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23liver>

Lung<https://secure-web.cisco.com/1XKYbStp6zlniQQGBzU4f0gduMoTau1U5yyvmIesFpsMaY3f2cR5j6W2QPc4GtGedxxshX7ptbCfKplO0o9jsZXiphZvcgpiPQtw-oOssga7ynnYD_CHbKQJeEM8_5u9TPFb19GG3FmqY4toeceqjmiWTSs0yfhfb2NmKWibHAvVLD0mPzLVwuATiO2V4M4n_saAoHzwxcEvHoD0e9eDI2Ii_FH792fHhpKa4iuGQLWTPgs67z92_tF0TG9cg0txvfG3_RcJmtAhNrWqOsRL0IW7_Iq-40zQuyLvXkf2ufaVnv_ejWxiBUUM9yUKu74VoarCpH9cO_EgAOOC6_CQPSZB96wocAG7UitNpx1VDv5I/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23lung>

Muscle<https://secure-web.cisco.com/1nsY7-8s6Mdq5cBxwOHeJssT5lSqxhlKgryxmg_rLOAIBAM-h2Vx5xavhHRzpvUUePZBYvPTqi11Ilf2NsVX7vaVrfBjLWSbsDg85VFuVJv2ooluTwULiiWpqljh78pqngicb0rJTWYb_-GKf48bvA76sjfHbgugOyi9o_dfmVCcFSmSHCxUoQbj-M3Kwni2kud7m-boaNuAlKxki5Hv2ngVC7tj5Oh9ysfixnaxdHvN1Mwgv8xRwo9GUIK9Coa60-oShCS-Mst7BM1HKyWRiQfs07YZk24xG8FWsr-gWUjY6bc0hArXhe0xDF-7F08dZbJZZNhEcGTkfPgrXxQlEoNGdasF226EnOx8uBzvh69o/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23muscle>

Nerve<https://secure-web.cisco.com/1XDdXE9PsX2kcHxh9XrPxCjsnI9bBtfoJTwIBr-X-yhNleBO64pyDK60Ep9cqumjUgM-dcP6ncqoNlEGhVE4OCK0sbRfqJ3l_WLX6yjgJ3MQu0sNflkvBET_Zx_aWX-cCqgMD3OXOoeoP0gDbwVfAUSJt_BlYtXCVYyyyU1qx5EyxNL_N0ngVwggdFcK6IjJgTYRQTkdkmYml89aZjjxCcTjTPgZ12S-eoGSNGPNd9xZnVpgOseSMcI9vjkqEqGcLa-MApJRA1_CU9g-ZX7tBGIwPvyHWoCmen-ZvMqs0YU8uWN6uyGCwuu7Ibrm5GHcfMT_-PwU5icgrdXUmvoqbYs2h4GRyNm0K79i7AYcsin8/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23nerve>

Prostate<https://secure-web.cisco.com/1OKCSdIG-FjetUP7sB9jxROl__lqxJgjXuosW82V3jVeWYewjv1pj4il7VoVcayyKSLmfazdEgIMmOKMxQSQWoOzMPt9jdkDXG1OaPfrthaagMjgEDO116NRr5hLQOOi8aE80l85q9qU2JM6ZY3cdzj2hs6EnvekE65kP2MbRm0YqyWoZ8IkwB6-4ol-S7noeyLXqh2JwkKuZ7vA6y9tYmo5g3C5yWvjK1mweKvIZH0zGRM-9cb6XYSfnVzB9v7W-Q9eZfw8dGCo5b9pLHRKol-b2h0S3s21dGXgDU0mN1ODnZrxRdbocu3NKsZvDbO1W9RkvvwlkXunYIoBM6hOv9PlH931g0eFTxhdCExv1NZ4/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23prostate>

 Endothelial cells<https://secure-web.cisco.com/1mMRjE0Es1bTPO3ZRVDc6aORwFFO2DOYZM4IeVhkNU23BVj_R-s_zuDFgU8AZDrEAt-rO7QDjPdX8mm3TywlHm_DzbD7XllKr6vJ5PZ7mrA_7fJS6hUJCRM6AaJxriac6VacA1jfKWvBmUQj53R1-UxgTLrmSq84PUG3laFw3QARRolRYkhbU5qFoef4KEi3_ZitYxh2yyZE-s7tsOORbqjpNudoXh3l3GaB7RMY1_vLOeOOoxmQZyXXhFboQ55GSkweDAtWxUeZiKh7WXyD4vhbODlYztGTUPxjuQIcyIEyfqkNMfXsiZaj8WYZinxP0RoEqwbrm8MQsLve1__O_Qyb7pRneBWa1pLhCjKFgqXw/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23endothelialcells>

Fibroblast<https://secure-web.cisco.com/1ME0Ff3dLTMszvkxzHTaisaA3AGSwV5QGnG5KvsY5WW8tt4gUBYqvsITNyaLYTqJGGgBU-ks4WXV6gaExNRtRIQrUxZq8HPLSmVr1T85VR1qjQDKjVlh9GG5MHV6hTx1itBcwV3nOoNl3mCu3N0fnMxFo_DtpLGWziE_YeABoxly7tRTkU6BepMN2atQ5Ds6tnPhhCtQEsoDZCGHoI8s1ScGsWGKQSrUNRPj8YpFlanWc0LVAmWTHebaUrABA68sAZ31txIBmX96VaIbhkIz_0sfW-4V0L6On2wnUpLGVMfZx4zaZNCabPp4Uxk7Z1CKsEMzFhiWwbNL-UebGQVw-s0fw5G_p1U0snXepCghXdtI/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23fibroblast>

Stem Cells<https://secure-web.cisco.com/1tcKIys0VRc2OUB_Vpsxz1_q5P-fyj3gvf2RXtgdwJCOCJmWlrNj3oGWSwjoh-EJXPQ9OPvAdjUQinYgh2rSLWgJLgaX28_HvAv991K4kjcRk2DDWjMlzlb_v_-ZUdMIkGlZ5IGXte7IXxvP84_g8ON1OnmS0sYPLJeWeoQKL8LXiGBenw0fLLMgh4_FjsVSeYJc3_6btfSRrT5ywr0HWDDuGDewe27Q2aU6Qe5wYBOHS6NTPpZTpbMH3_c47woFp04rBri8iNGwT3HXTuTHnkQAZhLnFIocP0lt0ALZ92_djRsu8SsU9jYg8GjpKIYofcC8aimt2iaQR5tqZFquRnjL5O7TNPkQ4ucvLOOMJU-k/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23stemcells>

Stromal Cells<https://secure-web.cisco.com/1wGdn4qsNcNpaqfpUC21tB3ekMu0AtrwmfCMYIMLYrJbGGxGB7yUd7fdbX1ew8J2hY90vf-1lxxSRC1TH-Y2blwdndYl3NaOPf41tTXzUKbeLpERJMX4MYOlg_3keeS0D28s0PeD4IOabTtOVOnK1FoiaQhNYdaamFP07698hYPIG876CvODsM6blN4nQ0KttmCURepdqO3JY9APnv0n4-92RnRdp0e8PKgMf0AwpSAiIIyRsO4P1PHT_F57RVEUYEayk-U49Re99ExW4w3QQvQhrG90mKD4eHv1zG_75nMTDjOaiIz6hvKttrsepQPG2KKXaQ8fvy5QXOBsOb5NxIDUyNJ6KQNGu4LXi4qZTqjM/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23stromalcells>


Cancer/Tumor<https://secure-web.cisco.com/1tZkuX74he1pPmG3tIXAUbFWtuQwKI_XK_cS0HX-AE3rFo7qU7IbSbEoM_7gyWJgxCaTKlzrgcR2kEXeRaLK9Z3786XKx7pgtQVuIwgmg-nk_z9IzDfzzYKeMqTmiwBIL_0zzhZZz0ZWxUBD1jQ_zN8xBfXMXiq7OdyxhAZxYi6-OVU0VHLItSzm4DnF3PEgsZ9utxdXzgHxVyQm4-Gb1CGJv_m2I7IYD8ATVTBKl6reLnc8U1XHqum2wp0XIjYPNeP7LjNpuaEpSVYgTSTOdvRe7v3pRCKOZP1OyXMlThVhB5BwAbZGw-57hQwcxqDSbGua2tKJH6XYHOdD5e-0iELgDr3EE6VMrkrULda0UoMI/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23cancer>

 Screening<https://secure-web.cisco.com/1iElg3OTCAONrTpECvnXZI-H5th_vx8IDex4B2kXLqFSG4kvlTN8z-FeuMcf9vIWIppnupq7xuhOnaZAxkBL6b4SyR0kAwU2w6qD1IxfudDera7ZB0NKO4IjHvxk0SIbMbO1xnLO7kNOJeEsTD7bhc83GF0EeJhXfBVsnud9gm2nSpFjpNgInVplkqiPvQrN80_UI9FdNFgosL8tRMvgmDHVG6_euHGWSXT13jrXU8n_8dG-IuA-eRK6tPIEb0DcJD2a27Qgs1Z0bHECoZUJRofbANsobYRaOmtsOXYhk8LIrJYyv5REOrUtAKFD8XuB80__i7W6mWPv4LtaKvoiovTmW3twztSw_X_H4ZmkZ6AA/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23screening>

3D bioprinting<https://secure-web.cisco.com/1iiuXREQBrZ-004WFtxqYaj-NuEd2HcKDSJRUTBJ9sKzp_9zFUZuBuja1YELOC1n7wvWpfCscqJJ30RA626e3cbnbu_hT3AhPWQfns7dPlZPeN3cH98iyeSE515ei_mlU47l_av7DMMtAILuBXzOpjPXNtwZm-9yvYKFZVgX3YRGUXF3bv5vRA1oXbyBx4wDmUnQDwfQg-C6vUvdQAeYe-wXuAEWt22BfragO8LJXRd0ZOq14p9HYL91-LvK4VavvG8OUFQgEtivUFKGuw2ULjzuB7YpUQVT55LyjLyBxXJCqE2nAhBvFofKof1wyMFS6Chmc02iswMUcvR31w_Xx16vy-lI5L69ZdFVT6mj2uxU/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%233dbioprinting>

 Imaging<https://secure-web.cisco.com/1N7Q8r-5Kv6eS47YzLE53diYCFV19OEfqu9tEhh783V2Is7MuJLEe1W_4dkO34sHT_ri5CfhQDfwypsjkpn56XrRDHj55r2G4HzNF1n2Sz2JS5u27zAuq5W0V2GhKz30WWq5mY9tH2wJii9VyxLXKNEBlA5pw0IzuO3-ljaD_ZCdlwjFk2cd8hhi7i7jZXfoRQ8l5jlzN5KzdabwUY2JuS04WbeeF8CzIRI4jerCXB1cy0ErcDpfp4uGkR29Cj6XEE1hR1Keot-2J58QWpthbgRC-BCDfMX6p5nVs8_GxvkzFRtAz7EjaeECxWqaaAQG1iInKvgaR-zjolCArG7ndGf_FG1Zlvi1lPmjJRlN2y1I/https%3A%2F%2Fnanohub.org%2Fgroups%2F3d3cfacility%2Fnews%23imaging>






________________________________

3D in Meetings


International Translational and Regenerative Medicine Conference
Date: 1st to 3rd November 2017
Location: Barcelona, Spain
Website: http://itmc.madridge.com/<http://secure-web.cisco.com/1FbExhs2hmDcHnJeGBt3SR2AUXW3SNg1pI-Ruw8MFQ5DZpqsiY22uyBduxbIl2jS44FsHgTgbRAxhRjMd2lpsTjmuDkeyii883qjGYXVBIrZT1UxQ5H9LoBDal0GJ5pBXIoZWhF2_42jX0QcD5fJn4bybaNaGq9YY4QY8V53KVRxkQ3hH_qdqztGepi3igwmDTmoXkbMCWdENrtFGuykScqkCaGc3Xho0e0wWJd0WPrcddf4FxZi40yGnJPTDHzSH2y7TIkgtCj_WCZUjlBPcBjfUCSr2kGY_a8N9cto_idkCaS5BQKUZIMsrKnXL9qlE5o_7WzRdJcb1bNLuKYMmMKh74lQKL51lyG_Iy7l3TSo/http%3A%2F%2Fitmc.madridge.com%2F>
Contact person: Samatha R


High-Content Analysis & 3D Screening
Date: 6th to 8th November 2017
Location: Cambridge, Massachusetts, USA
Website: HighContentAnalysis.com <http://secure-web.cisco.com/1oGLemnayF4-7_ubFkVuRpw9JjhVeqAtaZwP2SHk0AwXHoJDeQQekFZty79oiMuupJtmlaUMoxijl-6PFnwpwwDpK01eUU-SkpaaHxJfhuvu-JvQN5p5akzdLf2LpsRvatGr1uOH5veM87EaKW9JR0puvrQoFvpzW_neuZjf5h77JnTwtovLNvl_qGYyo379TJO9UUo-2ZoxyaTD9CWLN0ffrUYx9CU5In7WjjX8yy8gwgA7n9wME1-k6IsEmWlQGvVBZ_dL9u65tR34-2WtSHyg-XYj_PmCH7npbj1PXYXqne4dgsiERIeU3izOHQrcm0sOc82bYMNHlP0nrTIH3g_VGYt_zpr0vFrDTS-NQ4TY/http%3A%2F%2Fwww.worldpreclinicalcongress.com%2F3D-Cellular-Models%2F>
Contact person: Jaime Parlee
Organized by Cambridge Innovation Institute


World Preclinical Congress Europe 2017
Date: 15th to 17th November 2017
Location: Lisbon, Portugal
Website: http://worldpreclinicaleurope.com<http://secure-web.cisco.com/1xYI8EOjFklSfZu_Z9a3LZKJA-dJ9EaeOa42v0NZRqPeRi2lN3BPTD7OBL3iplPtrApXHeIxiphxDNF59pOMc9U_vc_zSdsVIo04SLOEJ4InloBsLiLSkxyFO_uNM4s1zJM_oEkoUQZDU5PNDAaX5c866Z825q8gXzZnGtsdG8cpQGd7FPNy12JWkmo2ycrSLdAknFnV6o9b0BVaRV0Wd0BHlLJwmoSWQmSvqftcWwv2-0sqRu4wGtLdY0zjLtJgzfnaEVo7zMBbuRzAv8ipTvY36nQ2p4mb_vmrY8uGffemzHBXlRCoc_tdfuVCbq_8YxWbPh4i8W1gQreoSXZopwlfTCKOR9l2YY48Lgt5BxiU/http%3A%2F%2Fworldpreclinicaleurope.com>
Contact person: Bethany Gray
Organized by: Cambridge Healthtech Institute


International Biotechnology and pharmaceutical Industry Forum
Date: 11th to 13th December 2017
Location: New Delhi, India
Website: http://biopharma-forum.com/#1<http://secure-web.cisco.com/1Z9_XRvYwcNDJdnI-eqwLTlQee9eCMzPkMs4X4HNe84B_J8R1uB3tkWYPLzBmT5G1FTPnWnk-j9sZxDOH62LhTmgir7Kufng1grOWKS8njCtlZsKRdBheOpLvyiH5wl-aeZUoTjexAXItL6kMsuDjPGve_bg3reKqGmloerYiO9xmdHbeoRtY-ptTgU9Ld_-9CQaG_UAjsuyZnPSw9qvZZg9pum0CdKnJa3_lqRj1MNZw4ra8gTjTGZMK5cp_OXPr75Bz0Zowhkml0Hm-CZ48_YY_ILljUKzVUJWehp8y4_e4AWKUBKvolhbTzAE76iUjCk6eL8uEomZ2u-bEqV24O9LAuc7Dq81EWwuHfh4KQUE/http%3A%2F%2Fbiopharma-forum.com%2F%231>
Contact person: sangavi
Organized by: Clyto access
Deadline for abstracts/proposals: 25th April 2017

ITMC-2017-Aims to discover advances in health practice & in relation to health disparities, as well as a breadth of other topics. Need for translational medicine, Challenges in translational medicine, Opportunities in translational medicine.


3D Cell Culture
Date: 21st to 22nd February 2018
Location: London, United Kingdom
Website: https://www.smi-online.co.uk/pharmaceuticals/uk/3D-Cell-Culture<https://secure-web.cisco.com/1YwIZW1hWzZbnyzO04k7NceUUBVGsboUvYrl0zmY35ctkhYG7xSz-n4Y4YDDRv-fTTzskaep92MtCkYokRMsSo2oSGV3BSTIQaJrcEV9_nv9l1nzwkNmBrYEo96m7XrY37q6qoHVMHmPsUTSEOcK7bzp3vcBZTUmzPRL5SJNX94EIT9wJU2zP9mOuzMdetwSO1pNR4tj9RimPEiQv94C0i6v_vZjmXeDYKrJ2MaiLB5fA-jwd2YCp8s6RGFnPIHjf6MhwXC4mK8GLeipILCFBkMpCo3kj3px2Rrc08mDCYje5BEAJCiFlO_m2gj23am3XlcgkOTuBvnk4Miwd83Y0VR0HD66ZIaGJMtE0_-eAR9Q/https%3A%2F%2Fwww.smi-online.co.uk%2Fpharmaceuticals%2Fuk%2F3D-Cell-Culture>
Contact person: Customer Services
Organized by: SMi group


Collaborative 3D Printing in Medical Practice
Date: 23rd to 25th February 2018
Location: Scottsdale, USA
Website: http://go.evvnt.com/153859-0<http://secure-web.cisco.com/1vJLOfn_ZEbA2joEY-1byWt9shm8mQH_xn_4byDmCeCx1I-DXIYiGvpq8zb25vNaP06QrScDXUJUEMBShWMV1kEpQeiFANMNyN7coFXwwIOuQROVUSKgd2KOscB9EGt8LNwo9hfNOauQTxOjAMVmTGGQc4Jr-Xkso7D-Tn21cNJDvvVDk62vJexS4UO7F6BAQ7fnBScWM4wjwtvQLhZFmHucVsbNsqQJ0BlDpiSK4f_Fb_bgvz96wukwDyIDBC52iVk-gQ9uxbZyzrAGEx6pnOk0aeT9Lf65XJUQe17CAh-icZG__Turk7u3lSnTAWs-mO-rQ5cJ1TYOm1OBfTdC8QyjBGDZwFWYlfo2mfXjAw99yHsI56gaWyHzU06YeMmoR/http%3A%2F%2Fgo.evvnt.com%2F153859-0>
Contact person: Department of Radiology CME Office
Organized by: Mayo Clinic
Collaborative 3D Printing in Medical Practice is designed to update and introduce radiologists, surgeons, dentists, and biomedical engineers on uses of 3D printing of anatomic models.


4th International Conference on Bio-based Polymers and Composites
Date: 2nd to 6th September 2018
Location: Balatonfüred, Hungary
Website: http://www.bipoco2018.hu<http://secure-web.cisco.com/1fPiBV7JFwJRIl5JST2qohmSaJ53vWQeFrC9S59VXw8dys1jmA-KkdGBwAYJEHSxiBHvPgt98V4lIbxl9QB1lkqRBZrOGGdCtEZWMuAPZEZkv8iqf90B2Bgrqufl6GlPSJQdITydp36RAi7Nqiyk8xqjrSLfi-BqRUQQAGkAxg8F_zwxFJWxPOjj_mZ5OGGxh5N7G3BV5nSjPnmeGV6yG0aB3tZkAbEQ_kyl8G8iRyEeD26tN1s7xlSZeWt-bZzhVgwGWBpQtO3LcbWFS2dTN3Ee28B8LSzkREp_nYDZ7J0CWgKY1Hi5imU6FfneVeH8DhLwdsZB6P72GgfOhB8t4dq_6imn0_x8QSq72CPbpKOM/http%3A%2F%2Fwww.bipoco2018.hu>
Contact person: Dóra Tátraaljai

Bio-based polymers and their blends, composites. Natural polymers and their modification. Natural fiber reinforced composites. Other raw materials based on natural resources. Biodegradation and environmental issues













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