Frontiers in Biophysics Seminar - Wed., Oct. 20
Event Date: | October 20, 2021 |
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Hosted By: | Department of Biological Sciences |
Time: | 1:30 p.m. |
Location: | DLR131 |
Priority: | No |
School or Program: | Non-Engineering |
College Calendar: | Show |
Title: From High-resolution Mechanisms to Genome Editing APplications for Type I CRISPR-Cas (CRISPR-Cas3)
Abstract: Six different types of CRISPR systems are utilized by microbes to form RNA-based immunity against mobile genetic elements. Type I CRISPR-Cas is the most widely distributed system. Its DNA interference mechanism is quite different from that of Cas9 and Cas12. Instead of introducing a single Double strand break (DSB) at the target site, Type I CRISPR-Cas shreds DNA into pieces in a multi-step process. This is done through the concerted actions of an RNA-buided target-searching complex called Cascade and a helicase-nuclease fusion enzyme Cas3. A total of six subtypes of Type I systems exist (I-A to I-E), each has unique mechanistic properties and may lead to distinct genome editing applications. I will present efforts from my group that lead to the high-resolution mechanistic understanding of several Type I CRISPR-Cas systems. I will further present data showing how we convert the mechanistic understanding to highly efficient deletion-editing tools (CRISPR-Cas3) in human cells.
Sponsors: Molecular Biophysics Training Program (MBTP) and Structural and Computational Biology and Biophysics (SCCB)
2021-10-20 13:30:00 2021-10-20 14:30:00 America/Indiana/Indianapolis Frontiers in Biophysics Seminar - Wed., Oct. 20 Ailong Ke, Professor of Molecular Biology and Genetics at Cornell University, will present this week's Frontiers in Biophysics Seminar on Wednesday, October 20, at 1:30 p.m. The seminar will be live-streamed from DLR 131. DLR131