BME Seminar - Wed., Feb. 17
Event Date: | February 17, 2016 |
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Hosted By: | Weldon School of Biomedical Engineering |
Time: | 9:30 a.m. |
Location: | MJIS 1001, WL campus |
Although pulling forces have been observed in axonal growth for several decades, their underlying mechanisms, absolute magnitudes, and exact roles are not well understood. Here, we quantified retrograde traction force in Aplysia Californica neuronal growth cones as they develop over time in response to a new adhesion substrate using two different experimental approaches. In the first approach, we developed a novel method for measuring traction forces using an atomic force microscope (AFM) with a cantilever that was modified with an Aplysia cell adhesion molecule (apCAM)-coated microbead. In the second approach, we used force-calibrated glass microneedles coated with apCAM ligands to guide growth cone advance. Both approaches showed that Aplysia growth cones can develop traction forces in the 102 nN range during adhesion-mediated advance. Interestingly, the absolute level of traction force did not correlate with growth cone advance towards the adhesion site, but the amount of microneedle deflection did. Our data support a hypothesis that adhesion complexes, which can undergo micron-scale elastic deformation, regulate the coupling between the retrogradely flowing actin cytoskeleton and apCAM substrates, stimulating growth cone advance if sufficiently abundant.
~BME Faculty Host: Fang Huang~
***Coffee and juice will be provided at West Lafayette***
2016-02-17 09:30:00 2016-02-17 10:30:00 America/Indiana/Indianapolis BME Seminar - Wed., Feb. 17 The Role of Traction Force in Neuronal Growth Cone Advance is the title of the seminar to be presented on Feb. 17th by Daniel M. Suter, Associate Professor of Biological Sciences at Purdue University. The seminar will be at 9:30 a.m. in MJIS 1001. MJIS 1001, WL campus